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Team:Rice University/STRATEGY
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=== Metabolic Parts === | === Metabolic Parts === | ||
| - | [[Image:TAL.png|right| | + | [[Image:TAL.png|right|200px|thumb|TAL monomer complexed with ''p''-coumaric acid. Active His is shown in black.]] |
*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=169745 Tyrosine Ammonia Lyase] (TAL) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and ''p''-coumaric acid. TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and ''trans''-cinnamic acid. The [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=36575&Template=fungiYeast ''Rhodotorula glutinis''] TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in ''Saccharomyces cerevisiae'' and ''Escherichia coli''. | *[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=169745 Tyrosine Ammonia Lyase] (TAL) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and ''p''-coumaric acid. TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and ''trans''-cinnamic acid. The [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=36575&Template=fungiYeast ''Rhodotorula glutinis''] TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in ''Saccharomyces cerevisiae'' and ''Escherichia coli''. | ||
[[Image:TAL catalysis.png|left|500px]] | [[Image:TAL catalysis.png|left|500px]] | ||
Revision as of 16:26, 29 October 2008
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SUMMARY ::: BACKGROUND ::: STRATEGY ::: CONSTRUCTS ::: RESULTS ::: ONGOING WORK ::: OUR TEAM ::: NOTEBOOK ::: GALLERY
SUMMARY ::: BACKGROUND ::: STRATEGY ::: CONSTRUCTS ::: RESULTS ::: ONGOING WORK ::: OUR TEAM ::: NOTEBOOK ::: GALLERY |
