Team:Rice University/STRATEGY

(Difference between revisions)

Revision as of 17:35, 29 October 2008

Tyrosine Ammonia Lyase (TAL) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and p-coumaric acid. TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and trans-cinnamic acid. The Rhodotorula glutinis TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in Saccharomyces cerevisiae and Escherichia coli. The p-coumaric acid produced by TAL will later be converted to resveratrol, while the trans-cinnamic acid will add a "floral" and "honey-like" bouquet to the beer.

Peanut STS monomer bound to resveratrol.

4-coumarate CoA ligase :: Stilbene synthase fusion protein (4CL:STS) - Completes the resveratrol biosynthetic pathway by catalyzing the conversion of p-coumaric acid to resveratrol, with a stoichiometry of 2:1. The 4CL:STS fusion protein was selected because it has been shown to more efficiently produce resveratrol than coexpression of the proteins separately (possibly due to substrate channeling).

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-[[Image:STS.png|right|190px|thumb|[http://www.rcsb.org/pdb/explore.do?structureId=1T6P STS] bound to resveratrol.]]
+[[Image:STS.png|right|190px|thumb|[http://www.rcsb.org/pdb/explore.do?structureId=1Z1F Peanut STS] monomer bound to resveratrol.]]
 *4-coumarate CoA ligase :: Stilbene synthase fusion protein (4CL:STS) - Completes the resveratrol biosynthetic pathway by catalyzing the conversion of ''p''-coumaric acid to resveratrol, with a stoichiometry of 2:1.  The 4CL:STS fusion protein was selected because it has been shown to more efficiently produce resveratrol than coexpression of the proteins separately (possibly due to substrate channeling).