Team:Rice University/STRATEGY

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=== Metabolic Parts ===
=== Metabolic Parts ===
[[Image:TAL.png|right|190px|thumb|[http://www.rcsb.org/pdb/explore.do?structureId=1T6P TAL] monomer.]]
[[Image:TAL.png|right|190px|thumb|[http://www.rcsb.org/pdb/explore.do?structureId=1T6P TAL] monomer.]]
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*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=169745 Tyrosine Ammonia Lyase] (TAL) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and ''p''-coumaric acid.  TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and ''trans''-cinnamic acid.  The [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=36575&Template=fungiYeast ''Rhodotorula glutinis''] TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in ''Saccharomyces cerevisiae'' and ''Escherichia coli''.  The ''p''-coumaric acid produced by TAL will later be converted to resveratrol, while the ''trans''-cinnamic acid will add a "floral" and "honey-like" bouquet to the beer.  
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*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=169745 Tyrosine Ammonia Lyase] (TAL) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K122010 BBa_K122010] - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and ''p''-coumaric acid.  TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and ''trans''-cinnamic acid.  The [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=36575&Template=fungiYeast ''Rhodotorula glutinis''] TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in ''Saccharomyces cerevisiae'' and ''Escherichia coli''.  The ''p''-coumaric acid produced by TAL will later be converted to resveratrol, while the ''trans''-cinnamic acid will add a "floral" and "honey-like" bouquet to the beer.  
[[Image:TAL catalysis.png|left|500px]]
[[Image:TAL catalysis.png|left|500px]]

Revision as of 21:14, 29 October 2008


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SUMMARY ::: BACKGROUND ::: STRATEGY ::: CONSTRUCTS ::: RESULTS ::: ONGOING WORK ::: OUR TEAM ::: NOTEBOOK ::: GALLERY

Contents

Metabolic Parts

TAL monomer.
  • Tyrosine Ammonia Lyase (TAL) BBa_K122010 - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and p-coumaric acid. TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and trans-cinnamic acid. The Rhodotorula glutinis TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in Saccharomyces cerevisiae and Escherichia coli. The p-coumaric acid produced by TAL will later be converted to resveratrol, while the trans-cinnamic acid will add a "floral" and "honey-like" bouquet to the beer.
TAL catalysis.png


















Peanut STS monomer bound to resveratrol.
  • 4-coumarate CoA ligase :: Stilbene Synthase Fusion Protein (4CL:STS) - Completes the resveratrol biosynthetic pathway by catalyzing the conversion of p-coumaric acid to resveratrol, with a stoichiometry of 2:1. The 4CL:STS fusion protein was selected because it has been shown to more efficiently produce resveratrol than coexpression of the proteins separately (possibly due to substrate channeling).















Circuit Design

Cellular Chassis

Fermentation Apparatus

SUMMARY ::: BACKGROUND ::: STRATEGY ::: CONSTRUCTS ::: RESULTS ::: ONGOING WORK ::: OUR TEAM ::: NOTEBOOK ::: GALLERY

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