Team:Rice University/STRATEGY

From 2008.igem.org

(Difference between revisions)
(→Metabolic Parts)
Line 19: Line 19:
=== Metabolic Parts ===
=== Metabolic Parts ===
[[Image:TAL.png|right|190px|thumb|[http://www.rcsb.org/pdb/explore.do?structureId=1T6P TAL] monomer.]]
[[Image:TAL.png|right|190px|thumb|[http://www.rcsb.org/pdb/explore.do?structureId=1T6P TAL] monomer.]]
-
*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=169745 Tyrosine Ammonia Lyase] (aka TAL, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K122010 BBa_K122010]) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and ''p''-coumaric acid.  TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and ''trans''-cinnamic acid.  The [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=36575&Template=fungiYeast ''Rhodotorula glutinis''] TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in ''Saccharomyces cerevisiae'' and ''Escherichia coli''.  The ''p''-coumaric acid produced by TAL will later be converted to resveratrol, while the ''trans''-cinnamic acid will add a "floral" and "honey-like" bouquet to the beer.  
+
*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=169745 Tyrosine Ammonia Lyase] (aka TAL, [http://partsregistry.org/wiki/index.php?title=Part:BBa_K122010 BBa_K122010]) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ''p''-coumaric acid and ammonia.  TAL also exhibits Phenylalanine Ammonia Lyase (PAL) activity, converting L-phenylalanine to ''trans''-cinnamic acid and ammonia.  Our work has focused on using [http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=36575&Template=fungiYeast ''Rhodotorula glutinis''] TAL because its ratio of TAL to PAL activity is high compared with other TAL homologs. In addition, previous studies have shown that this enzyme can be expressed as a functional protein in ''Saccharomyces cerevisiae'' and ''Escherichia coli''.  While the ''p''-coumaric acid produced by TAL will serve as a substrate for resveratrol biosynthesis, the ''trans''-cinnamic acid is expected to add a "floral" and "honey-like" bouquet to the beer.  
[[Image:TAL catalysis.png|left|500px]]
[[Image:TAL catalysis.png|left|500px]]

Revision as of 22:09, 29 October 2008


justify justify


GradientBar.jpg

ProjectTitle.jpg

OUR TEAM  ::: SUMMARY ::: INTRODUCTION ::: STRATEGY ::: RESULTS ::: ONGOING WORK ::: GALLERY

Contents

Metabolic Parts

TAL monomer.
  • Tyrosine Ammonia Lyase (aka TAL, BBa_K122010) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to p-coumaric acid and ammonia. TAL also exhibits Phenylalanine Ammonia Lyase (PAL) activity, converting L-phenylalanine to trans-cinnamic acid and ammonia. Our work has focused on using Rhodotorula glutinis TAL because its ratio of TAL to PAL activity is high compared with other TAL homologs. In addition, previous studies have shown that this enzyme can be expressed as a functional protein in Saccharomyces cerevisiae and Escherichia coli. While the p-coumaric acid produced by TAL will serve as a substrate for resveratrol biosynthesis, the trans-cinnamic acid is expected to add a "floral" and "honey-like" bouquet to the beer.
TAL catalysis.png


















Peanut STS monomer bound to resveratrol.
  • 4-coumarate CoA ligase :: Stilbene Synthase Fusion Protein (aka 4CL:STS, BBa_K122005) - Completes the resveratrol biosynthetic pathway by catalyzing the conversion of p-coumaric acid to resveratrol, with a stoichiometry of 2:1. The 4CL:STS fusion protein was selected because it has been shown to more efficiently produce resveratrol than coexpression of the proteins separately (possibly due to substrate channeling).
4CL STS catalysis.png












Circuit Design

Cellular Chassis

Fermentation Apparatus

SUMMARY ::: BACKGROUND ::: STRATEGY ::: CONSTRUCTS ::: RESULTS ::: ONGOING WORK ::: OUR TEAM ::: NOTEBOOK ::: GALLERY

Retrieved from "http://2008.igem.org/Team:Rice_University/STRATEGY"