Team:Rice University/STRATEGY
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*Our design goal was to construct a circuit that would propagate through several generations without a selection pressure and be highly expressed during all stages of fermentation. To address these goals we constructed three expression cassettes that, when concatenated, would integrate genomically into a highly transcribed locus, have an inducible selectable marker, and express the resveratrol pathway highly under anaerobic conditions. | *Our design goal was to construct a circuit that would propagate through several generations without a selection pressure and be highly expressed during all stages of fermentation. To address these goals we constructed three expression cassettes that, when concatenated, would integrate genomically into a highly transcribed locus, have an inducible selectable marker, and express the resveratrol pathway highly under anaerobic conditions. | ||
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*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122012 Cassette 1] - anaerobically induced expression of 4CL:STS | *[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122012 Cassette 1] - anaerobically induced expression of 4CL:STS | ||
*#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122000 pPGK1] - serves the dual purpose of 5' homologous recombination region, and anaerobically induced promoter. | *#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122000 pPGK1] - serves the dual purpose of 5' homologous recombination region, and anaerobically induced promoter. | ||
*#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122005 4CL:STS] - converts ''p''-coumaric acid and 3 malonyl-CoA molecules to resveratrol. | *#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122005 4CL:STS] - converts ''p''-coumaric acid and 3 malonyl-CoA molecules to resveratrol. | ||
*#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122003 tCYC1] - bi-directional transcriptional stop. | *#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122003 tCYC1] - bi-directional transcriptional stop. | ||
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*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122015 Cassette 2] - inducible expression of Bleocin resistance. | *[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122015 Cassette 2] - inducible expression of Bleocin resistance. |
Revision as of 00:29, 30 October 2008
OUR TEAM ::: SUMMARY ::: INTRODUCTION ::: STRATEGY ::: RESULTS ::: ONGOING WORK
OUR TEAM ::: SUMMARY ::: INTRODUCTION ::: STRATEGY ::: RESULTS ::: ONGOING WORK |