Team:Rice University/STRATEGY
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[[Image:circuit.png|right|350px|thumb|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122020 BBa_K122020].]] | [[Image:circuit.png|right|350px|thumb|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122020 BBa_K122020].]] | ||
*Our design goal was to construct a circuit that would propagate through several generations without a selection pressure and be highly expressed during all stages of fermentation. To address these goals we constructed three expression cassettes that, when concatenated, would integrate genomically into a highly transcribed locus, have an inducible selectable marker, and express the resveratrol pathway highly under anaerobic conditions. | *Our design goal was to construct a circuit that would propagate through several generations without a selection pressure and be highly expressed during all stages of fermentation. To address these goals we constructed three expression cassettes that, when concatenated, would integrate genomically into a highly transcribed locus, have an inducible selectable marker, and express the resveratrol pathway highly under anaerobic conditions. | ||
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*#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122003 tCYC1] - bi-directional transcriptional stop. | *#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122003 tCYC1] - bi-directional transcriptional stop. | ||
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+ | *[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122015 Cassette 2] - inducible expression of Bleocin resistance. | ||
+ | *#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122001 pGAL1 TetO ZeoR] - provides galactose inducible, TetR repressible, Zeocin/Bleocin resistance. This part will allow us to select for recombinants. | ||
+ | *#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K122004 tADH1] - bi-directional transcriptional stop. Strategically placed downstream of Zeor and upstream of pADH1 to prevent erroneous recombination at those regions. | ||
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*Cassette 3 - constitutive expression of TAL | *Cassette 3 - constitutive expression of TAL |
Revision as of 00:32, 30 October 2008
OUR TEAM ::: SUMMARY ::: INTRODUCTION ::: STRATEGY ::: RESULTS ::: ONGOING WORK
OUR TEAM ::: SUMMARY ::: INTRODUCTION ::: STRATEGY ::: RESULTS ::: ONGOING WORK |