Team:Rice University/STRATEGY

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SUMMARY ::: BACKGROUND ::: STRATEGY ::: CONSTRUCTS ::: RESULTS ::: ONGOING WORK ::: OUR TEAM  ::: GALLERY

Contents

Metabolic Parts

TAL monomer.
  • Tyrosine Ammonia Lyase (aka TAL, BBa_K122010) - Begins the resveratrol biosynthetic pathway by catalyzing the the conversion of L-tyrosine to ammonia and p-coumaric acid. TAL also has activity as Phenylalanine Ammonia Lyase (PAL) via conversion of L-phenylalanine to ammonia and trans-cinnamic acid. The Rhodotorula glutinis TAL was selected specifically because of its relatively high TAL/PAL activity ratio, and its successful expression in Saccharomyces cerevisiae and Escherichia coli. The p-coumaric acid produced by TAL will later be converted to resveratrol, while the trans-cinnamic acid will add a "floral" and "honey-like" bouquet to the beer.
TAL catalysis.png


















Peanut STS monomer bound to resveratrol.
  • 4-coumarate CoA ligase :: Stilbene Synthase Fusion Protein (aka 4CL:STS, BBa_K122005) - Completes the resveratrol biosynthetic pathway by catalyzing the conversion of p-coumaric acid to resveratrol, with a stoichiometry of 2:1. The 4CL:STS fusion protein was selected because it has been shown to more efficiently produce resveratrol than coexpression of the proteins separately (possibly due to substrate channeling).
4CL STS catalysis.png












Circuit Design

Cellular Chassis

Fermentation Apparatus

SUMMARY ::: BACKGROUND ::: STRATEGY ::: CONSTRUCTS ::: RESULTS ::: ONGOING WORK ::: OUR TEAM ::: NOTEBOOK ::: GALLERY

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