Team:The University of Alberta/11 July 2008

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Chris

  • Sequenced inserts of Blue Ox in J61003 Transf.2 and Transf.4. Will run them up to MBSU for sequencing on Monday.
  • Set up O/N cultures of Tansf.2 and Transf.4 for minipreps; volunteers will miniprep tomorrow.

Tom

  • Yesterday's transformation is weird, the "digest" plate has lots of growth...huh, maybe our enzyme is not working good enough

Winnie

  • did blast on sequencing J61003 with 23, 25, 35 and 99

except 99 everything matched,yes!

  • sequenced Tom's I0500 +35 and 99 using reverse primer
  • made LB+kan plates. Volunteers on saturday, please put them in the -4c
  • took pictures of gel for Tom and Chris, Tom's gel is in the firdge.
    • Chris's gel is not as expected, so left in the fridge till Monday to ask Chris. EDIT: Gel was fine!