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Team:The University of Alberta/12 June 2008
From 2008.igem.org
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*Set up O/N of Purple Russian, Blue Ox and Tryp in their original pUC57 plasmids. | *Set up O/N of Purple Russian, Blue Ox and Tryp in their original pUC57 plasmids. | ||
*Ran colony PCR of the colonies used above, to make sure that they are positive colonies with our genes. | *Ran colony PCR of the colonies used above, to make sure that they are positive colonies with our genes. | ||
| + | *Made 15ml of Kan and 100(!)ml of Amp - should last us a long time | ||
Revision as of 19:44, 12 June 2008
Today
- Made up new Tet and Kan plates because the ones we made forever ago were made with 1/5 the correct amount of Antibiotics in them!
- Set up O/N of Purple Russian, Blue Ox and Tryp in their original pUC57 plasmids.
- Ran colony PCR of the colonies used above, to make sure that they are positive colonies with our genes.
- Made 15ml of Kan and 100(!)ml of Amp - should last us a long time
