Team:The University of Alberta/12 June 2008

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Meeting At 4:00PM!
==Today==
==Today==
*Made up new Tet and Kan plates because the ones we made forever ago were made with 1/5 the correct amount of Antibiotics in them!
*Made up new Tet and Kan plates because the ones we made forever ago were made with 1/5 the correct amount of Antibiotics in them!

Revision as of 19:45, 12 June 2008

Meeting At 4:00PM!

Today

  • Made up new Tet and Kan plates because the ones we made forever ago were made with 1/5 the correct amount of Antibiotics in them!
  • Set up O/N of Purple Russian, Blue Ox and Tryp in their original pUC57 plasmids.
  • Ran colony PCR of the colonies used above, to make sure that they are positive colonies with our genes.
  • Made 15ml of Kan and 100(!)ml of Amp - should last us a long time