Team:The University of Alberta/13 August 2008
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(New page: '''Saima''' *Ran out of LacIERE minipreps. Set up the O/N's of LacIERE using colonies form original Transformant stocks.) |
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'''Saima''' | '''Saima''' | ||
*Ran out of LacIERE minipreps. Set up the O/N's of LacIERE using colonies form original Transformant stocks. | *Ran out of LacIERE minipreps. Set up the O/N's of LacIERE using colonies form original Transformant stocks. | ||
+ | *Digested RBS+TetR with Eco/XbaI. | ||
+ | |||
+ | '''Chris''' | ||
+ | *Jason and I began the site-directed mutagenesis of the binary vector. Its going to take a loooong time (over 7 hours for the PCR alone) so we are going to finish it (i.e. do the digestion with DpnI and transform) tomorrow. |
Latest revision as of 21:11, 13 August 2008
Saima
- Ran out of LacIERE minipreps. Set up the O/N's of LacIERE using colonies form original Transformant stocks.
- Digested RBS+TetR with Eco/XbaI.
Chris
- Jason and I began the site-directed mutagenesis of the binary vector. Its going to take a loooong time (over 7 hours for the PCR alone) so we are going to finish it (i.e. do the digestion with DpnI and transform) tomorrow.