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Team:The University of Alberta/13 June 2008
From 2008.igem.org
Today
- Developed the Westerns after an overnight exposure. Blank yet again. James thought the problem was due to the development solution used - it was an old tube in the fridge that had been mixed months ago and was probably no good anymore. New solution was made and applied, film was exposed for 60 seconds and again, there were no results. This is odd because there were obvious thick bands of overexpressed proteins when we did the Coomassie staining. We will need to set up some cultures when we use the Ni-NTA columns so perhaps we will do them in duplicate and use one set for Westerns again.
