Team:The University of Alberta/15 July 2008

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Today

  • Got sequencing back from Blue Ox in J61003 Transf.2 and Transf. 4. Both of them look good; Transf.2 looks "better" though because it has fewer mismatches.
  • Did PCR on Tryp again because no one can remember if it had been done successfully with the newest primers.
    • Ran portion of PCR out on 1% gel to confirm product. PCR purified the rest.
  • Transformed the I0500+new vector ligation
  • The BioBricks we had ordered for synthesis have arrived (minus one of them)! Siama has transformed them following the protocol supplied by the company.
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