Team:The University of Alberta/17 June 2008

From 2008.igem.org

Revision as of 20:37, 17 June 2008 by Cwk (Talk | contribs)

Today

Today we are continuing the troubleshooting from yesterday.

Troubleshooting Results

Saima
Tried doing digests from Jason's digests of the genes in pUC57 (see below) using columns from three different kits. The results were all similar - very low concentrations for each. Next we are going to try running already purified DNA of a known, high concentration through the columns and measure the concentration that is eluted to determine how much, if any, is sticking to the column
Jason

June17 puc57 genes.jpg
  • The digests of the reporter genes in pUC57 were run on a 1% agarose gel. The bands were the correct size; see the image to the right.
  • Making a single cut with Xba in the J61003 vector to attempt to ligate it back together and thereby testing our ligation buffer and enzyme. This will let us know if the reason our transformations havent been working is due to bad ligase buffer/enzyme (eg, maybe the ATP in the ligase buffer is no good anymore).
  • also redoing the Purple Russian J61003 ligation using new ligation buffer

Chris

  • Continuing with the westerns. Made some new destain solution which should last for a while.

Tom

  • Made stocks of TetR and I0500 from the iGEM Parts Binder

Plant Project

Our first batch of plants are almost flowering on schedual with the Six week schedual we set for them A new tray was planted to day and put into the growth chamber