Team:The University of Alberta/26 August 2008

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Today

  • The P0380+MCS transformants grew! Making overnights today.
  • The mutated PR grew as well. Overnights were set up.
  • Redoing the Site Directed Mutagenesis as follows:
    • 5 reactions
      • i) Mutagenesis Control
      • ii) NotI-1B rxn
      • iii) NotI-2B rxn
      • iv) NotI-3B rxn
      • v) Rxn with all 3 primers
  • Minipreps! Lots and lots of minipreps!
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