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Team:The University of Alberta/26 May 2008
From 2008.igem.org
NK 130-530
Hey guys, Kelly and I (Anthony) were in on Friday and we managed to excise the bands and do the gel purification. Kelly has put Winnie's digested producs in the -20, I have put the purification products also in the -20 (wrapped together in green tape and labelled). As well, anything that was left out on the bench was put in the -20 because we have no clue what was in those tubes. Kelly and I were having a difficult time following the lab book, so PLEASE be explicitly clear about what has been performed, what is in each of the biobricks, etc... It might seem annoying and redundant but the volunteers have simply no way of following otherwise. A simple summary of each procedure and protocol would be GREATLY appreciated! We didn't have time to do to the sequencing reaction because Kelly has left for Rocky Mountain, and I am not about to start a 2.5 hr reaction at 7:30 PM on a Friday night!
Good luck with things on Monday, and I will see you Tuesday
Anthony
Contents |
Today In The Lab
- Overday's of Purple Russian and Tryp
- Finish Fundraising Letter
- Finish Construction of the Binary Vector
- Plant 2 more trays of arabidopsis
- Work with the Butanol Pathway some more
Jason:
I have made overday's of Blue Ox as well as Purple Russian and Tryp.
Volunteers Scheduled for Today
5-8 AS
5-8 KR
5-8 SG
Volunteers
- If you could would please make Glycerol stocks as well as a mini prep on those Overdays that Jason has done
A Note To Everyone
There are boxes on the left hand side of the -20 freezer in the lab for our ligations, digestions, mini-preps and random other things. They are labelled "iGEM Digestions 1", etc. If you have anything to store in the freezer, please place items in the appropriate boxes. This makes finding what we want in the freezer a whole lot easier than sifting through racks upon racks of tubes. Don't forget to write down in the lab book (or online) which box you put everything in and where in each box you placed it (we've tried to set up a grid-like numbering system for each box [A1, A2, etc for each space]).
