Team:The University of Alberta/27 June 2008

From 2008.igem.org

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==Today==
==Today==
*The O/Ns that were set up last night turned out well. Did mini-preps on them today.  
*The O/Ns that were set up last night turned out well. Did mini-preps on them today.  
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*The transformations done yesterday turned out perfectly. Now [[:Image:june27_transformants|''this'']] is what transformations should look like!
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*The transformations done yesterday turned out perfectly. Now [[:Image:N120400274_33714509_398.jpg|''this'']] is what transformations should look like!
*Running colony PCR as a test for contamination to explain the strange results we got yesterday
*Running colony PCR as a test for contamination to explain the strange results we got yesterday
*Gel purified ligations of all the parts we were missing in J61003 (21, 22, 25, 35, 99); also purifying J61003 because we have run out!
*Gel purified ligations of all the parts we were missing in J61003 (21, 22, 25, 35, 99); also purifying J61003 because we have run out!
*More transformations: this time on the parts listed above in J61003
*More transformations: this time on the parts listed above in J61003
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*Made SDD-PAGE gels to run the purified His-tagged proteins on.
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*Made PAGE gels to run the purified His-tagged proteins on.

Latest revision as of 17:07, 3 July 2008

Today

  • The O/Ns that were set up last night turned out well. Did mini-preps on them today.
  • The transformations done yesterday turned out perfectly. Now this is what transformations should look like!
  • Running colony PCR as a test for contamination to explain the strange results we got yesterday
  • Gel purified ligations of all the parts we were missing in J61003 (21, 22, 25, 35, 99); also purifying J61003 because we have run out!
  • More transformations: this time on the parts listed above in J61003
  • Made PAGE gels to run the purified His-tagged proteins on.