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Team:The University of Alberta/29 July 2008
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(New page: ==The Big Whoops== We found the source of the problems we have been having with using NgoMIV and AgeI: the ER0x and BisDx parts dont have the NgoMIV and AgeI sites in them! Not good! We ma...)
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(New page: ==The Big Whoops== We found the source of the problems we have been having with using NgoMIV and AgeI: the ER0x and BisDx parts dont have the NgoMIV and AgeI sites in them! Not good! We ma...)
Newer edit →
Revision as of 17:08, 29 July 2008
The Big Whoops
We found the source of the problems we have been having with using NgoMIV and AgeI: the ER0x and BisDx parts dont have the NgoMIV and AgeI sites in them! Not good! We may have to get them resynthesized! But first we will:
- Check ER01 and ER02 for toxicity to determine if we need to have one or both remade
Today
Chris
- Was going to do colony PCR on the transformants from the weekend but someone put the plates in the incubator instead of the fridge! Now they're overgrown and no good :( So....
- I will do PCR on the original ligation using Vf and Vr to determine whether or not Tryp was ligated into J6. If it was then...
- Retransform the ligation and do colony PCR again tomorrow
- I will do PCR on the original ligation using Vf and Vr to determine whether or not Tryp was ligated into J6. If it was then...
