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Team:The University of Alberta/3 June 2008
From 2008.igem.org
Continued from yesterday...
Yesterday, Jason made transformants using our working Blue Ox (used the primers, ligated the product into vector and transformed competent cells). The ligation didnt work well (or the transformation efficency was far too low), because we got only a single colony on the 400ul plate. Nevertheless, a single colony is all that is needed to set up an overday culture. The volunteers that come in this evening will have to do a miniprep on this culture.
