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Team:The University of Alberta/3 October 2008
From 2008.igem.org
(Difference between revisions)
(New page: James **transformed tom's ligation from yesterday, incubate overnight in the 37degree **ligate the digest (21+23)(x/p) and (24+25) (s/p) form 9/10/08 to to vector and incubate at 37 ove...) |
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| - | James | + | *James |
| - | **transformed tom's ligation from yesterday, | + | **transformed tom's ligation from yesterday,incubate overnight in the 37degree |
| - | + | **ligate the digest (21+23)(x/p) and (24+25) (s/p) form 9/10/08 to to vector and incubate at 37 overnight | |
| - | **ligate the digest (21+23)(x/p) and (24+25) (s/p) form 9/10/08 to to vector | + | |
| - | and incubate at 37 overnight | + | ==David== |
| + | i miniprepped yesterdays cultures and checked them with a NotI digest. | ||
| + | |||
| + | pTET+BisdA worked | ||
| + | pTET+BisdB worked | ||
Latest revision as of 03:39, 29 October 2008
- James
- transformed tom's ligation from yesterday,incubate overnight in the 37degree
- ligate the digest (21+23)(x/p) and (24+25) (s/p) form 9/10/08 to to vector and incubate at 37 overnight
David
i miniprepped yesterdays cultures and checked them with a NotI digest.
pTET+BisdA worked pTET+BisdB worked
