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Team:The University of Alberta/5 August 2008
From 2008.igem.org
(Difference between revisions)
(New page: ==Back to the Lab after the Long Weekend!== '''Chris''' *Winnie redid the ligation of J6 and Tryp. I put transforming this on hold though because... **She also did colony PCR on some more ...) |
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*Winnie redid the ligation of J6 and Tryp. I put transforming this on hold though because... | *Winnie redid the ligation of J6 and Tryp. I put transforming this on hold though because... | ||
**She also did colony PCR on some more of the previous transformants. Got the same result as the two times I did it before; bands ~600bp. It might be GFP - if it is, then it means the digest of J6 I originally did didn't work. I'm doing PCR on the digest to check; if I get a band it means the digest didnt work and that explains the odd results. Im also doing PCR on some undigested GFP to compare and determine if it is GFP or some other fragment. | **She also did colony PCR on some more of the previous transformants. Got the same result as the two times I did it before; bands ~600bp. It might be GFP - if it is, then it means the digest of J6 I originally did didn't work. I'm doing PCR on the digest to check; if I get a band it means the digest didnt work and that explains the odd results. Im also doing PCR on some undigested GFP to compare and determine if it is GFP or some other fragment. | ||
| + | '''Saima''' | ||
| + | *Did Colony PCR on Col.4, 5 & 6 of I0500+ER01 and I0500+ER02.Set up the O/N's for each of these colonies. Streaked for single colonies | ||
| + | **Ran them on 1%gel. ER02 band size is not the right one. | ||
Revision as of 18:46, 6 August 2008
Back to the Lab after the Long Weekend!
Chris
- Winnie redid the ligation of J6 and Tryp. I put transforming this on hold though because...
- She also did colony PCR on some more of the previous transformants. Got the same result as the two times I did it before; bands ~600bp. It might be GFP - if it is, then it means the digest of J6 I originally did didn't work. I'm doing PCR on the digest to check; if I get a band it means the digest didnt work and that explains the odd results. Im also doing PCR on some undigested GFP to compare and determine if it is GFP or some other fragment.
Saima
- Did Colony PCR on Col.4, 5 & 6 of I0500+ER01 and I0500+ER02.Set up the O/N's for each of these colonies. Streaked for single colonies
- Ran them on 1%gel. ER02 band size is not the right one.
