Team:UCSF/Synthetic Chromatin Properties

From 2008.igem.org

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     <h2 align="justify">Analysis of our Data</h2>
     <h2 align="justify">Analysis of our Data</h2>
     <p align="justify">Single-cell analysis was done using flow-cytometry. Flow cytometry measurements were taken using a BD LSR-II flow cytometer (BD Biosciences). For each sample, 10,000 cells were counted, and GFP fluorescence was measured by exciting at 488 nm with a 100 mW Coherent Sapphire laser. Cells that were positive for GFP are seen in the microscope images on the lower right panel  and their predicted population distribution is shown in the green curve in the ficticious graph in the left (below).</p>
     <p align="justify">Single-cell analysis was done using flow-cytometry. Flow cytometry measurements were taken using a BD LSR-II flow cytometer (BD Biosciences). For each sample, 10,000 cells were counted, and GFP fluorescence was measured by exciting at 488 nm with a 100 mW Coherent Sapphire laser. Cells that were positive for GFP are seen in the microscope images on the lower right panel  and their predicted population distribution is shown in the green curve in the ficticious graph in the left (below).</p>
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     <p align="center"><img src="https://static.igem.org/mediawiki/2008/2/2b/Analysis_data.jpg" width="750" height="458" /></p>
     <p align="justify">&nbsp;</p>
     <p align="justify">&nbsp;</p>
     <h2 align="justify">The Properties of Our Synthetic Chromatin Bit</h2>
     <h2 align="justify">The Properties of Our Synthetic Chromatin Bit</h2>
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     <h3 align="justify">1. Targeting of Sir2 leads to complete silencing of reporter</h3>
     <h3 align="justify">1. Targeting of Sir2 leads to complete silencing of reporter</h3>
     <p align="justify">text here</p>
     <p align="justify">text here</p>
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     <p align="justify">&nbsp;</p>
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     <p align="justify">&nbsp;</p>
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     <p align="justify">Similar results were obtained for other promoters (e.g. Fig1 P, see below).</p>
     <p align="justify">Similar results were obtained for other promoters (e.g. Fig1 P, see below).</p>
     <h3 align="justify">2. Dominant over Transcription Factors</h3>
     <h3 align="justify">2. Dominant over Transcription Factors</h3>
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       <p align="justify">text here</p>
       <p align="justify">text here</p>
       <p align="justify">&nbsp;</p>
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       <p align="center">&nbsp;</p>
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     <p align="justify">&nbsp;</p>
     <p align="justify">&nbsp;</p>

Revision as of 21:21, 28 October 2008

Minusgalactose pluspheromone.png Plusgalactose minuspheromone.png Pheromone R.png Regionalsilencing.png Regionalsilencing R.png Memory R1.png Memory R2.png


Untitled Document

Design of our System (previous)

 

Synthetic Chromatin Bit (Part II)

 

Why use Chromatin as a Tool for Synthetic Biology?

text here

Analysis of our Data

Single-cell analysis was done using flow-cytometry. Flow cytometry measurements were taken using a BD LSR-II flow cytometer (BD Biosciences). For each sample, 10,000 cells were counted, and GFP fluorescence was measured by exciting at 488 nm with a 100 mW Coherent Sapphire laser. Cells that were positive for GFP are seen in the microscope images on the lower right panel and their predicted population distribution is shown in the green curve in the ficticious graph in the left (below).

 

The Properties of Our Synthetic Chromatin Bit

We studied how our yeast synthetic chromatin system behaves...

 

1. Targeting of Sir2 leads to complete silencing of reporter

text here

 

RESULT 1:

 

Similar results were obtained for other promoters (e.g. Fig1 P, see below).

2. Dominant over Transcription Factors

text here

 

 

 

RESULT 2:

 

text here

3. Regional Silencing

text here

 

RESULT 3:

text

 

4. Binary

text here

 

5. Memory

text here

RESULT 5:

 

 

text here

 

Higher-Order Systems (next)




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