Team:UNIPV-Pavia/Notebook/Week10

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Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7
Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Week 14



Week 10: 07/21/08 - 07/25/08

07/21/08

  • Colony PCR for BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079: 6 colonies from single colonies plate.
BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079
  • Gel results: all the 6 picked colonies were true positive! we chose the 5th colont to grow a 9 ml overnight culture.
  • We also infected 9 ml LB + Amp with 30 µl of BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040 and BBa_B0030-BBa_C0061 glycerol stocks to grow two overnight cultures.
  • Ligation:
    • BBa_J23100-BBa_B0030-BBa_C0012-BBa_B1006
    • BBa_B0030-BBa_I15009-BBa_BBa_B1006-BBa_R0082
    • BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-BBa_R0062
    • BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006
  • We incubated ligations at 16°C overnight.