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Team:University of Chicago/Papers
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''[http://www.sciencedirect.com/science/article/B6TWB-4NMWRCS-F/1/9587d2845309a42beb00a6663a1b5085 Practical recombinant hybrid mussel bioadhesive fp-151].'' A recombinant fp-1/fp-5 fusion protein works in cell bioadhesion assays. 1g/L production and soluble. ---Steve Kron | ''[http://www.sciencedirect.com/science/article/B6TWB-4NMWRCS-F/1/9587d2845309a42beb00a6663a1b5085 Practical recombinant hybrid mussel bioadhesive fp-151].'' A recombinant fp-1/fp-5 fusion protein works in cell bioadhesion assays. 1g/L production and soluble. ---Steve Kron | ||
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| + | ====Yeast/Tyrosinase==== | ||
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| + | ''[http://www.jcb.org.proxy.uchicago.edu/cgi/reprint/131/4/895 The Golgi-localization of Yeast Emp47p Depends on Its Di-lysine Motif But Is Not Affected by the retl-1 Mutation in oL-COP].'' --Jata | ||
====Other==== | ====Other==== | ||
Revision as of 23:31, 4 June 2008
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Please post links to relevant papers here with brief descriptions. Sign your name in case we have questions. I'll start us off with everything I've gotten by email so far.
Contents |
Expression
Patent: Method of producing bioadhesive protein. Making mussel proteins since 1990! ---Steve Kron
Patent: Bioadhesive precursor protein expression vectors. I swear these people just photocopied their application and mailed it out again. ---Steve Kron
Patent: Production of bioadhesive precursor protein analogs by genetically engineered organisms. New pictures this time. ---Steve Kron
Secretion
An E. coli Expression System for the Extracellular Secretion of Barley α-Amylase (pdf). Entry into the wide-word of secretion-system manipulation.
Type III protein secretion systems in Bacterial Pathogens of Plants and Animals (pdf). A nice review of natural type III secretion systems we can take advantage of, preferably without getting bubonic plague.
Salmonella Secretion pathway
Cloning and Transfer of the Salmonella Pathogenicity Island 2 Type III Secretion System for Studies of a Range of Gram-Negative Genera (pdf). Wilson, et al.
Cellular microbiology of intracellular Salmonella enterica: functions of the type III secretion system (pdf). Kuhle, et al.
A conserved amino acid sequence directing intracellular type III secretion(pdf). Miao, et al.
Mgfp-5
Recombinant mussel adhesive protein Mgfp-5 as cell adhesion biomaterial. Modified Mgfp-5 is better than Cell-Tak or poly-L-lysine; unmodified is about the same as Cell-Tak. It survives harsher washes on glass than BSA or Cell-Tak. ---Steve Kron
Practical recombinant hybrid mussel bioadhesive fp-151. A recombinant fp-1/fp-5 fusion protein works in cell bioadhesion assays. 1g/L production and soluble. ---Steve Kron
Yeast/Tyrosinase
Other
2007 Ideas. Great big honking list of references. ---Thomas Graham
"Quorum Sensing in E.Coli" Bioluminescence Activation via Q.S. -Rob M
"[1]." Patent for stable extracellular tyrosinase which may or may not be useful --Laura
