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Team:Warsaw/Calendar-Main/12 August 2008
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| + | <center><h4>Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, iompt)</center>Piotr:</h4> | ||
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| + | Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in <i>E. coli</i> Rosetta strain. | ||
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| + | <p>1. Isolation of plasmids from cultures inocluated on previous day.<br> | ||
| + | 2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains). | ||
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| + | <h3>Checking for presence of A on the cell membrane</h3> | ||
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| + | <h4>Piotr</h4> | ||
| + | <p>Inoculation of omp_A_alfa, omp_Z_alfa and omp_omega_A_alfa (with and without induction).</p> | ||
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Revision as of 14:12, 12 October 2008
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Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, iompt)Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in E. coli Rosetta strain. 1. Isolation of plasmids from cultures inocluated on previous day. Checking for presence of A on the cell membranePiotrInoculation of omp_A_alfa, omp_Z_alfa and omp_omega_A_alfa (with and without induction).
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