Team:Warsaw/Calendar-Main/12 August 2008

From 2008.igem.org

(Difference between revisions)
Line 4: Line 4:
<html>
<html>
-
 
-
 
-
<center><h4>Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, iompt)</center>Piotr:</h4>
 
-
<br>
 
-
Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in <i>E. coli</i> Rosetta strain.
 
-
 
-
<p>1. Isolation of plasmids from cultures inocluated on previous day.<br>
 
-
2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains). 
 
-
</p>
 
-
 
-
 

Revision as of 14:24, 12 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Checking for presence of A on the cell membrane

Piotr

Inoculation of omp_A_alfa, omp_Z_alfa and omp_omega_A_alfa (with and without induction).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/12_August_2008"