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Team:Warsaw/Calendar-Main/12 August 2008
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PPrzanowski (Talk | contribs) (New page: {{WarNotebook}} <!-- do not edit above me! --> Testy na oporność dawaną przez białko w pożywce: Piotr Sprawdzenie jaka minimalna ilość białka dodana do pożywki daje oporność...) |
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| - | + | <h3>Checking for presence of protein A on the cell membrane</h3> | |
| - | + | <h4>Emilia</h4> | |
| + | <p>Inoculation of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>pACYC177+OmpA_omega_ΔA_alpha</a> (with and without induction).</p> | ||
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| + | <h3>Purification of proteins: Z-alpha and Z-omega</h3><h4>Piotr</h4> | ||
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| + | <p><ol><li>Bacteria centrifuged and sonicated (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</li> | ||
| + | <li>Sonicate centrifuged. </li> | ||
| + | <li>Supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20.</li> | ||
| + | </ol></p> | ||
| + | <br> | ||
| + | </html> | ||
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Latest revision as of 19:52, 25 October 2008
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Checking for presence of protein A on the cell membraneEmiliaInoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_Z_alpha and pACYC177+OmpA_omega_ΔA_alpha (with and without induction). Purification of proteins: Z-alpha and Z-omegaPiotr
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