Team:Warsaw/Calendar-Main/12 August 2008

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<h4>Emilia</h4>
<h4>Emilia</h4>
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<p>Inoculation of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-deltaA-alpha>pACYC177+OmpA_&Delta;A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>pACYC177+OmpA_omega_&Delta;A_alpha</a> (with and without induction).</p>
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<p>Inoculation of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>pACYC177+OmpA_omega_&Delta;A_alpha</a> (with and without induction).</p>

Latest revision as of 19:52, 25 October 2008

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Checking for presence of protein A on the cell membrane

Emilia

Inoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_Z_alpha and pACYC177+OmpA_omega_ΔA_alpha (with and without induction).

Purification of proteins: Z-alpha and Z-omega

Piotr

  1. Bacteria centrifuged and sonicated (according to protocol).
  2. Sonicate centrifuged.
  3. Supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20.

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