Team:Warsaw/Calendar-Main/12 August 2008

From 2008.igem.org

Revision as of 14:12, 12 October 2008 by MKrzyszton (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, iompt)

Piotr:
Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in E. coli Rosetta strain.

1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains).

Checking for presence of A on the cell membrane

Piotr

Inoculation of omp_A_alfa, omp_Z_alfa and omp_omega_A_alfa (with and without induction).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/12_August_2008"