Team:Warsaw/Calendar-Main/15 October 2008

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<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a></h3>
<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a></h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<p> Inoculation of colonies from plate with ligation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + AraC+pBAD+AID to LB with ampicillin. </p>
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<p> Inoculation of colonies from plate with ligation of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> + <a href=http://partsregistry.org/Part:BBa_K103002>AID under pBAD/araC (BBa_K103002)</a> to LB with ampicillin. </p>

Revision as of 21:39, 27 October 2008

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Preparation of linker_alpha (BBa_K103009)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 + linker_alpha (BBa_K103009).

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr, Michał K.

  1. Repetition of ligation of isolated DNA fragments: pACYC177 + OmpA-linker-omega-linker (BBa_K103016) (from 30 September).
  2. Transformation of TOP10 with above ligation.
  3. Plating on LB with kanamycin.

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

Inoculation of colonies from plate with ligation of pSB1A3 + AID under pBAD/araC (BBa_K103002) to LB with ampicillin.

Preparation of OmpA_linker_alpha_linker under Plac (BBa_K103017)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3_pLac_OmpA_ + alpha2 with kanamycin.

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