Team:Warsaw/Calendar-Main/20 June 2008

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(Difference between revisions)
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<tr><th>Product</th><th>Template</th><th>Primers</th><th>Product length</th></tr>
<tr><th>Product</th><th>Template</th><th>Primers</th><th>Product length</th></tr>
<tr><th>linker-A</th><td>pDRIVE-TapTag</td><td>AL+link10+homo2 + AP+NotI </td><td>470 bp</td></tr>
<tr><th>linker-A</th><td>pDRIVE-TapTag</td><td>AL+link10+homo2 + AP+NotI </td><td>470 bp</td></tr>
-
<tr><th>alpha-linker</th><td>pUC19</td><td>AlphaL+SacI + AlphaP+link10+homo2 </td><td>1022 bp</td></tr>
+
<tr><th>alpha-linker</th><td>pUC19</td><td>AlphaL+SacI + AlphaP+link10+homo2 </td><td>600 bp</td></tr>
-
<tr><th>omega-linker</th><td>pUC19</td><td>OmegaL+SacI + OmegaP+link10+homo2 </td><td>779 bp</td></tr>
+
<tr><th>omega-linker</th><td>pUC19</td><td>OmegaL+SacI + OmegaP+link10+homo2 </td><td>400 bp</td></tr>
</table>
</table>

Revision as of 14:28, 8 October 2008

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Michał L., Ewa, Marcin

PCR
ProductTemplatePrimersProduct length
linker-ApDRIVE-TapTagAL+link10+homo2 + AP+NotI 470 bp
alpha-linkerpUC19AlphaL+SacI + AlphaP+link10+homo2 600 bp
omega-linkerpUC19OmegaL+SacI + OmegaP+link10+homo2 400 bp

Universal PCR program for A, alpha and omega

TemperatureTime
94°C4:00
94°C0:3028 cycles
50°C0:45
72°C2:00
72°C10:00
4°Cinfinite

Polymerase: Pfu-Turbo