Team:Warsaw/Calendar-Main/31 August 2008

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<li>0.5 ml of each liquid culture was plated on solid LB supplemented with 200 ug/ml rifampicin + Tetracycline</li>
<li>0.5 ml of each liquid culture was plated on solid LB supplemented with 200 ug/ml rifampicin + Tetracycline</li>
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<li>0.1 ml of each liquid culture was plated on solid CTAXI medium</li>
 
<li>Plasmids were isolated from the rest of cultures.</li>
<li>Plasmids were isolated from the rest of cultures.</li>
<li>Gel electrophoresis of iolated plasmids and gel-out of pZC320</li>
<li>Gel electrophoresis of iolated plasmids and gel-out of pZC320</li>

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Blue-white screening and rifampicin test in GM2163

Michał L., Ewa

  1. 0.5 ml of each liquid culture was plated on solid LB supplemented with 200 ug/ml rifampicin + Tetracycline
  2. Plasmids were isolated from the rest of cultures.
  3. Gel electrophoresis of iolated plasmids and gel-out of pZC320
  4. Gel-outed plasmids were transformed to Top10 cells

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