Team:Warsaw/Calendar-Main/8 August 2008

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Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_A_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>OmpA_A_alpha</a> with and without inductor (0,5 mm/mL IPTG) in <i>E. coli</i> Rosetta strain. </p>
Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_A_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>OmpA_A_alpha</a> with and without inductor (0,5 mm/mL IPTG) in <i>E. coli</i> Rosetta strain. </p>
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<h3>Purification of proteins: Z-alpha and Z-omega</h3><h4>Emilia</h4>
 
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<p>100 ml of medium inoculated with overnight culture, then used as inoculum for 1 l culture with presence of ampicillin, kanamycin and IPTG (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</p>
 
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Revision as of 21:18, 19 October 2008

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Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, ompt)

Piotr:

Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5 mm/mL IPTG) in E. coli Rosetta strain.




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