Team:Warsaw/Calendar-Main/8 August 2008

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<h3>Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, ompt) </h3> <h4>Piotr:</h4>
<h3>Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, ompt) </h3> <h4>Piotr:</h4>
<p>
<p>
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Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_A_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>OmpA_A_alpha</a> with and without inductor (0,5 mm/mL IPTG) in <i>E. coli</i> Rosetta strain. </p>
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Inoculation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha>OmpA_omega_&Delta;A_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-deltaA-alpha>OmpA_&Delta;A_alpha</a> with and without inductor (0,5 mm/mL IPTG) in <i>E. coli</i> Rosetta strain. </p>

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Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, ompt)

Piotr:

Inoculation of OmpA_omega_ΔA_alpha and OmpA_ΔA_alpha with and without inductor (0,5 mm/mL IPTG) in E. coli Rosetta strain.