Team:Warsaw/Calendar-Main/9 June 2008

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<html><h3>Preparation of pMPMT5-AID+AIDT7 construct</h3>
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<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID%2BAID-T7>pMPMT5-AID+AIDT7</a> construct</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of confirmed plasmid pMPMT5-AID+AID-T7 with HindIII and SalI (2x Tango buffer).</li>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of confirmed plasmid <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID%2BAID-T7>pMPMT5-AID+AID-T7</a> with HindIII and SalI (2x Tango buffer).</li>
<li>DNA gel electrophoresis.</li>
<li>DNA gel electrophoresis.</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Isolation of DNA</a> from proper band (3300 bp).</li>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Isolation of DNA</a> from proper band (3300 bp).</li>

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Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

  1. Digest of confirmed plasmid pMPMT5-AID+AID-T7 with HindIII and SalI (2x Tango buffer).
  2. DNA gel electrophoresis.
  3. Isolation of DNA from proper band (3300 bp).
  4. Sequencing of isolated band with AIDseqP and T7Rseq.

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