Team:iHKU/Notebook

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==Wet lab log book for the biobrick subgroup==
==Wet lab log book for the biobrick subgroup==

Revision as of 13:51, 4 July 2008


Home The Team The Project Parts Submitted to the Registry Modeling Notebook


Wet lab log book for the biobrick subgroup

(Many Thanks to MIT team for setting a good example for us ^_^)

  • Click on any day below to see what wet-lab procedures were conducted.
  • log book summary

0703 done: punched: and transformed. BBa_Q04510 BBa_R0062 BBa_R0011 BBa_I732096

Planning and summary section for the biobrick subgroup

here is the Biobrick subgroup progress,

  • Roy has got these following biobircks:

Part:BBa_E0240 Colony standard measurement for promotor

p1010 psb3K3

  • parts already obtained by peter：

Part:BBa_F2622 Colony

Part:BBa_T9002 Punch

Part:BBa_I731007 Punch

Part:BBa_B0030 Punch

S17 strain colony ready for ccdB


  • parts we further need to get

Part:BBa_Q04510 1013 7A Transf

Part:BBa_R0062 1000 4G Transf

Part:BBa_R0011 1000 4b Transf

BBa_I732096 1014 3G Transf reporter containing the standard GFP and LacZa

  • parts that may be useful

http://partsregistry.org/Part:BBa_B0031

http://partsregistry.org/Part:BBa_B0032

http://partsregistry.org/Part:BBa_B0033

http://partsregistry.org/Part:BBa_B0034


  • parts that we need to build:

1 Part:BBa_K094001CheZ assumed without rbs

2 Part:BBa_K094002LuxRI assumed with rbs and terminator


  • composite we need to construct:

1plaLuxRpluxCheZGFPLva BBa_F2622 BBa_B0030 BBa_K094001 BBa_B0030 BBa_E0022 BBa_B0015 （something wrong with GFPLva and i will change them later）

2plaLuxRpluxCIpldCheZGFPLva BBa_F2622 BBa_Q04510 BBa_B0030 BBa_K094001 BBa_E0430

3plaLuxRpluxLacZ BBa_F2622 BBa_B0030 BBa_I714901 BBa_B0015

4plaLuxRpluxCIpldLacZ BBa_F2622 BBa_Q04510 BBa_B0030 BBa_I714901 BBa_B0015

5plaLuxRIpluxCheZGFPLva BBa_R0011 BBa_K094002 BBa_R0062 BBa_B0030 BBa_K094001 BBa_E0430

6 first

7 Part:BBa_K094009

Notebook

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.

Find more information on how to use the calendar feature by going to the general calendar page.

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