Virginia/8 July 2008

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(New page: ==Goals== *Prepare overnight broth of successful single colony of Promoter + RBS transformation *Plate Screening plasmid (psb1a10) when it arrives from iGem *Try again with ligation of RFP...)
 
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==Goals==
==Goals==
-
*Prepare overnight broth of successful single colony of Promoter + RBS transformation
+
*Prepare overnight broth of single successful colony of Promoter + RBS transformation
*Plate Screening plasmid (psb1a10) when it arrives from iGem
*Plate Screening plasmid (psb1a10) when it arrives from iGem
*Try again with ligation of RFP and GFP enzyme cut DNA
*Try again with ligation of RFP and GFP enzyme cut DNA
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*Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation
*Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation
*Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon
*Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon
-
 
==Notes==
==Notes==
-
*Maxiprepped  bba_B0015 and bba_B0034 smell strongly of alcohol, we are dubious of the content of the prepared DNA
+
*Maxiprepped  B0015 and B0034 smell strongly of alcohol, we are dubious of the content of the prepared DNA
 +
==Accomplished==
 +
*Starting from maixprepped DNA we cut:
 +
**E0240, I715039, B0034 (dubious, see above), BP1
 +
*Short Ligations: (45min)
 +
**Test Vector 1: I715039 + E0240
 +
***Did a ligation each for non-purified DNA (after digestion) and purified DNA.
 +
**B0034 (RBS) + BP1
 +
***Only non-purified post-digestion DNA
 +
**Plated all short ligations
 +
*Long Ligations: (overnight @ room temp)
 +
**Test Vector 1: I715039 + E0240 (unpurified)
 +
**B0034 (RBS) + BP1
 +
*Plated screening plasmid [http://partsregistry.org/Part:pSB1A10 pSB1A10] that we received today
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[[Team:Virginia/Notebook|Notebook]]
+
[[Team:Virginia/Notebook|Back to Notebook]]

Latest revision as of 22:19, 8 July 2008

Goals

  • Prepare overnight broth of single successful colony of Promoter + RBS transformation
  • Plate Screening plasmid (psb1a10) when it arrives from iGem
  • Try again with ligation of RFP and GFP enzyme cut DNA
  • Transform this ligation and pray that it grows in the incubator
  • Run a gel of linearized and cut DNA of BP1 and terminator assembly to verify transformation
  • Begin ligation of standard RBS with BP1 gene to begin parallel assembly of our final operon

Notes

  • Maxiprepped B0015 and B0034 smell strongly of alcohol, we are dubious of the content of the prepared DNA

Accomplished

  • Starting from maixprepped DNA we cut:
    • E0240, I715039, B0034 (dubious, see above), BP1
  • Short Ligations: (45min)
    • Test Vector 1: I715039 + E0240
      • Did a ligation each for non-purified DNA (after digestion) and purified DNA.
    • B0034 (RBS) + BP1
      • Only non-purified post-digestion DNA
    • Plated all short ligations
  • Long Ligations: (overnight @ room temp)
    • Test Vector 1: I715039 + E0240 (unpurified)
    • B0034 (RBS) + BP1
  • Plated screening plasmid [http://partsregistry.org/Part:pSB1A10 pSB1A10] that we received today


Back to Notebook