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Beijing Normal/7 October 2008
From 2008.igem.org
PCR restrict digestion of T7 termitor and its vector was performed. We use double digestion system, BamH1 and XbaI in NEB Buffer 3. Xba1 was added more than BamH1. ligation was performed later. We cannot do the transformation today because there is no Top10 left in the fridge.
