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EPF-Lausanne/9 July 2008
From 2008.igem.org
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This morning, we find a problem in our system :
The Las/Lux problem
We found that there was a problem of cross-talking between Las and Lux, the two quoring systems we wanted to use. After a documental research by Bart and Sebastian, we will try to determine wether it is possible to use RHLR instead of Las (Lux is fixed by the Ron Weiss system). Some papers might show that there is no cross-talking between RHLR and Las, and therefore between RHLR and Lux.
We have just replace the Las by Rhl in our general scheme.
We have prepared some buffers : SOC, LB, SOB. See the protocols in the protocol page.
