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Edinburgh/8 August 2008
From 2008.igem.org
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Week 8
Friday 8 August 08
- Transformation of L32 (pSB1A2+crtB+crtI) on plates 98-99. (HX)
- Plate 94 (BABEL2+glgC-mut1,2,3) has white colonies, so can be prepped for minipreps. Plate 95 (pSB1A2+glgC-mut1,2) has blue colonies. Because of this, transformation of L31 (glgC-mut1,2 to Edinbrick1) has been repeated on plates 100-101. (HX)
- Digested PcstA (P54) and Edinbrick1 using Spe1/EcoR1. Ligated as L33, to be transformed tomorrow (OG)
- Arabidopsis thaliana isa1 and isa2 cDNAs have arrived as well as Zea mays su1, iso2 and iso3. The Arabidopsis genes are full of forbidden restriction sites, so it would be best to use the maize genes. Primers will need to be designed. (AH)
- PCRed cenA and cex with a) KOD polymerase, b) Velocity polymerase separately to make P55 (cenA by KOD), P56 (cenA by Velocity), P57 (cex by KOD) and P58 (cex by Velocity). (Yan)
- Ligated rbs+dxs with rbs+crtE as L34 (pSB1A2+dxs+crtE) and rbs+dxs with rbs+lims1 as L35 (pSB1A2+dxs+lims1). (Yan)
- M79 sequencing results indicate that M79 is BABEL2+crtE. (AH)
