x
OD600
Calibration Method
- An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.)
- Dilutions were plated out at 10-5 and 10-6 fold dilutions, and colonies were counted using a colony counter to determine the total number of cells in the original dilutions.
Results
| Relative Cell Density
| OD600 Reading
| Colonies on x100,000 dilution
|
| 1
| 1.6483
| 745
|
| 0.8
| 1.4913
|
| 0.6
| 1.1058
|
| 0.4
| 1.0167
| 308
|
| 0.2
| 0.5885
|
| 0.1
| 0.2982
|
| 0.08
| 0.2567
|
| 0.06
| 0.1910
|
| 0.04
| 0.1208
|
| 0.02
| 0.070
|
| 0.01
| 0.0346
|
x
|
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