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Illinois/19 July 2008
From 2008.igem.org
LAB WORK
Receptor Tyrosine Kinase method
glycerol added to 10%, cells in freezer
Transformation:
1. cells put in ice 30 min with 1μL plasmid(100ng)
2. Heat Shock for 2 minutes at 42 degrees
3. ice for 8 minutes
4. add cells to 1μL LB
5. grow for 1 hour
6. Plate 100 to 200 μL on LB and amp, grow overnight
