15 August 2008
Wet Lab
Cloning
- Plates form yestreday's transformations checked.
- J31005, B0015, J04650 and I13401 transformants grew overnight but no growth was registered for the C0012 transformant nor the controls.
- PCR primers for XylR, LacI and AmyE integration sequences designed and sent for synthesis
B.subtilis
- The transformation protocol 2 (click link here for the protocol) was performed yesterday. The transformed cells have not yet grown on the LB agar plates containing 100ug/ul streptinomycin. We will leave over the weekend to grow further.
|