15 October 2008
Wet Lab
Digestion of minipreps
- Today minipreps were prepared. These were digested and run on a gel to confirm the presence of the correct constructs. The folowing constructs were minipreped: Gene art 14 (pXyl-spoVG) and 5' EpsE integration sites.
- To test that insert with the correct size is present in the plasmid, the minipreps were digestd with EcoRI and PstI. The image below shows the results:
- Several of construct 14 appear to have succssfully been ligated. In addition all of the 5'EpsE appear to have worked.
- To construct 5'EpsE integration site we used a PCR product and as a result of the approach, there is a possibility that the orientation of this biobrick in the vector is incorrect. Digestion with XbaI SpeI will confirm correct orientation.
- If the PCR product has the correct orientation then the insert will fall out with this digestion. Clearly only 5'EpsE-1 is correct.
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