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Imperial College/17 September 2008
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Biofabricator Subtilis
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Why B. subtilis?
B. subtilis: Benefits vs Challenges
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Example Application:
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17 September 2008
Wet Lab
O.D.
600
vs Colony Forming Units
The protocol to obtain the calibration curve of O.D.
600
vs Colony Forming Units was performed today. The protocol can be found on this link
[1]
. The following O.D.
600
and dilutions were used:
O.D
600
of 0.5 = x10
-6
and x10
-7
O.D
600
of 1 = x10
-6
and x10
-7
O.D
600
of 1.5 = x10
-6
and x10
-7
O.D
600
of 2 = x10
-6
and x10
-7
O.D
600
of 2.5 = x10
-6
and x10
-7
O.D
600
of 3 = x10
-7
and x10
-8
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