Imperial College/22 July 2008

From 2008.igem.org

June
MTWTFSS
            [http://2008.igem.org/wiki/index.php?title=Imperial_College/1_June_2008&action=edit 1]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/2_June_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Imperial_College/3_June_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Imperial_College/4_June_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Imperial_College/5_June_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Imperial_College/6_June_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Imperial_College/7_June_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Imperial_College/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/9_June_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Imperial_College/10_June_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Imperial_College/11_June_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Imperial_College/12_June_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Imperial_College/13_June_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Imperial_College/14_June_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Imperial_College/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/16_June_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Imperial_College/17_June_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Imperial_College/18_June_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Imperial_College/19_June_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Imperial_College/20_June_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Imperial_College/21_June_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Imperial_College/22_June_2008&action=edit 22]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/23_June_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Imperial_College/24_June_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Imperial_College/25_June_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Imperial_College/26_June_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Imperial_College/27_June_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Imperial_College/28_June_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Imperial_College/29_June_2008&action=edit 29]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/30_June_2008&action=edit 30]
July
MTWTFSS
  [http://2008.igem.org/wiki/index.php?title=Imperial_College/1_July_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Imperial_College/2_July_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Imperial_College/3_July_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Imperial_College/4_July_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Imperial_College/5_July_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Imperial_College/6_July_2008&action=edit 6]
[http://2008.igem.org/Imperial_College/7_July_2008 7] [http://2008.igem.org/Imperial_College/8_July_2008 8] [http://2008.igem.org/Imperial_College/9_July_2008 9] [http://2008.igem.org/wiki/index.php?title=Imperial_College/10_July_2008&action=edit 10] [http://2008.igem.org/Imperial_College/11_July_2008 11] [http://2008.igem.org/wiki/index.php?title=Imperial_College/12_July_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Imperial_College/13_July_2008&action=edit 13]
[http://2008.igem.org/wiki/index.php?title=Imperial_College/14_July_2008&action=edit 14] [http://2008.igem.org/Imperial_College/15_July_2008 15] [http://2008.igem.org/Imperial_College/16_July_2008 16] [http://2008.igem.org/wiki/index.php?title=Imperial_College/17_July_2008&action=edit 17] [http://2008.igem.org/Imperial_College/18_July_2008 18] [http://2008.igem.org/wiki/index.php?title=Imperial_College/19_July_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Imperial_College/20_July_2008&action=edit 20]
[http://2008.igem.org/Imperial_College/21_July_2008 21] [http://2008.igem.org/Imperial_College/22_July_2008 22] [http://2008.igem.org/Imperial_College/23_July_2008 23] [http://2008.igem.org/Imperial_College/24_July_2008 24] [http://2008.igem.org/Imperial_College/25_July_2008 25] [http://2008.igem.org/wiki/index.php?title=Imperial_College/26_July_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Imperial_College/27_July_2008&action=edit 27]
[http://2008.igem.org/Imperial_College/28_July_2008 28] [http://2008.igem.org/Imperial_College/29_July_2008 29] [http://2008.igem.org/Imperial_College/30_July_2008 30] [http://2008.igem.org/wiki/index.php?title=Imperial_College/31_July_2008&action=edit 31]
August
MTWTFSS
        [http://2008.igem.org/Imperial_College/1_August_2008 1] [http://2008.igem.org/Imperial_College/2_August_2008 2] [http://2008.igem.org/wiki/index.php?title=Imperial_College/3_August_2008&action=edit 3]
[http://2008.igem.org/Imperial_College/4_August_2008 4] [http://2008.igem.org/Imperial_College/5_August_2008 5] [http://2008.igem.org/wiki/index.php?title=Imperial_College/6_August_2008&action=edit 6] [http://2008.igem.org/Imperial_College/7_August_2008 7] [http://2008.igem.org/Imperial_College/8_August_2008 8] [http://2008.igem.org/wiki/index.php?title=Imperial_College/9_August_2008&action=edit 9] [http://2008.igem.org/Imperial_College/10_August_2008 10]
[http://2008.igem.org/Imperial_College/11_August_2008 11] [http://2008.igem.org/Imperial_College/12_August_2008 12] [http://2008.igem.org/Imperial_College/13_August_2008 13] [http://2008.igem.org/Imperial_College/14_August_2008 14] [http://2008.igem.org/Imperial_College/15_August_2008 15] [http://2008.igem.org/Imperial_College/16_August_2008 16] [http://2008.igem.org/Imperial_College/17_August_2008 17]
[http://2008.igem.org/Imperial_College/18_August_2008 18] [http://2008.igem.org/Imperial_College/19_August_2008 19] [http://2008.igem.org/Imperial_College/20_August_2008 20] [http://2008.igem.org/Imperial_College/21_August_2008 21] [http://2008.igem.org/Imperial_College/22_August_2008 22] [http://2008.igem.org/wiki/index.php?title=Imperial_College/23_August_2008&action=edit 23] [http://2008.igem.org/Imperial_College/24_August_2008 24]
[http://2008.igem.org/Imperial_College/25_August_2008 25] [http://2008.igem.org/Imperial_College/26_August_2008 26] [http://2008.igem.org/Imperial_College/27_August_2008 27] [http://2008.igem.org/Imperial_College/28_August_2008 28] [http://2008.igem.org/Imperial_College/29_August_2008 29] [http://2008.igem.org/wiki/index.php?title=Imperial_College/30_August_2008&action=edit 30] [http://2008.igem.org/Imperial_College/31_August_2008 31]

22 July 2008

Debriefing

Each Group gave a summary of their sections, followed by a discussion on any potential problems:

B.subtilis

  • The group has found an amazing database of promoters and encoding regions of the B.subtilis genome. Several promoters have been investigated, the group was unclear if new terminators other than the E.coli terminators will be required.

Light Induction

  • The natural light detecting pathway has been investigated and the relevant parts required have been investigated. In addition, parameters for the modelling have been investigated including response time.
  • The only remaining issue is to try to engineer a light detecting pathway in B.suntilis.

Biomaterials

  • Secretion paths have been investigated by the biomaterials group. The main transport system used in B.suntilis is the SRP transport system. The signal peptide used is a variable sequence, however several signal peptides are commonly used. The group has suggested using 3 signal peptides in parallel with the 3 proposed biomaterials leading to 9 potential constructs, however after discussion the group agreed it would be better to use just one signal peptide for each biomaterial leading to just 3 potential constructs.
  • One key unresolved question is how the signal peptide is cleaved, needs to be resolved


  • Biomaterials have been chosen to pursue, these include a range of naturally occurring and synthetic peptides. The group discussed about using the mass spectrometry facilities at Imperial to confirm the presence of the biomaterial and levels of other secreted proteins.

Summary

  • Key issue is to work out a strategy for assembly of our constructs.



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