Las

From 2008.igem.org

Las/Rhl cell signaling system

Responsible: Robert Cool, Alicia Allen, and Erin Feeney

Las System

Signal Molecule: An AHL called PAI-1 (N-3-oxododecanoyl-l-hsl)(3-oxo-C12-hsl)

Bacterial species: Pseudomonas aeruginosa gram(-) possibly E.coli (see article 3)

Receiver protein: LasR

Effect of binding: TXN activation of virulence genes, lasA, lasB, apr, toxR

Synthase: LasI enzyme

Target Genes: lasI, lasA, lasB, apr, toxR

Regulation: unknown

References

"Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes"

JP Pearson, EC Pesci and BH Iglewski [http://jb.asm.org/cgi/reprint/179/18/5756?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&titleabstract=las&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT]

"Regulation of Pseudomonas Quinolone Signal Synthesis in Pseudomonas aeruginosa"

Dana S. Wade, M. Worth Calfee, Edson R. Rocha, Elizabeth A. Ling, Elana Engstrom, James P. Coleman, and Everett C. Pesci [http://jb.asm.org/cgi/content/full/187/13/4372?view=long&pmid=15968046 2]

Posttranscriptional Control of Quorum-Sensing-Dependent Virulence Genes by DksA in Pseudomonas aeruginosa

Florence Jude,Thilo Köhler,Pavel Branny,Karl Perron,Matthias P. Mayer,Rachel Comte, and Christian van Delden [http://jb.asm.org/cgi/content/full/185/12/3558?view=long&pmid=12775693 3]

Pending: [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1447470]

Rhl System

Signal Molecule: An AHL called PAI-2, Plasminogen activator inhibitor-2, N-butanoyl-homoserine lactone (C4HSL)

Bacterial species: Pseudomonas aeruginosa, gram(-)

Receiver Protein: Rhl R

Effect of Binding: activation of Rhamnosyl Transferase, then making RL (rhamnolipid)

Synthase: RhlA and RhlB

Target Genes: pqsABCDE and phnAB

Regulation: unknown

References

[http://jb.asm.org/cgi/reprint/189/13/4827 background information on Las and Rhl]

Parts Needed:

LasR + pro/term

RhlR + pro/term

RhlI + pro/term

pqsABCDE + pro/term

pqsR

pqsH + pro/term

phnAB

LasI

RBS