Minnesota/26 July 2008

From 2008.igem.org

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1. Plate growth successful: Colonies grew on the plates made 07-25-08. 2mL cultures of 10 colonies were made in LB broth. Numbering goes as follows: 1. Lac I 1, 2. Lac I, 3. Lac I, 4. BV:Lac #2, 5. BV:Tet #1, 6. BV:Tet #2, 7. BV:Tet/p22 #1, 8. BV:Tet/p22 #1, 9. BV:Tet/p22, 10. BV:Tet/p22 #2.
2. Plasmid prep 10 cultures: Prepping using the QIA buffering prep, extract the DNA from cultures 1-10. Place in -20C O/N as to not denature the DNA.
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