Purdue/18 July 2008

From 2008.igem.org

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mCherry bacteria are STILL not red. Looked up on Clontech (website selling mCherry)--found that maybe antibodies are necessary to visualize?

I'm thinking it was a bad assumption to make that mCherry would make bacteria red in visible light just like mRFP-1. I emailed Katie Clifford my problem.

From several books, it has been documented that LacZ takes around 2-5 minutes to be transcribed into beta-galactosidase. Still need to find time it takes to diffuse from the cell and cleave X-gal. Perhaps is negligible? Miller (1972) details an experiment about timing gene expression using beta-gal.

Edited by Janie Stine