Purdue/18 September 2008

From 2008.igem.org

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Digested SOS plasmids:

  • Mixture:
    • 40uL DNA
    • 5uL NEB Buffer 2 (10x)
    • 13uL ddH2O
    • 0.5uL BSA
    • 1uL EcoRI
    • 1uL SpeI
  • Mixed, stored at 37C for 1 hour
  • Placed in -20C to halt reaction

We'll run it through a gel and purify the SOS and LacZ parts soon. Then ligation, transformation, testing!

Edited by Janie Stine